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KMID : 0357119940160040357
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Korean Journal of Immunology
1994 Volume.16 No. 4 p.357 ~ p.364
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Purification of Human Papillomavirus Type 18E6 Recombinant Protein and Usage for Serodiagnosis
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진승원
강주현/손우익/유왕돈/정태화/박순희
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Abstract
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Human papillomavirus type 16 and 18 encode two major transforming proteins E6 and E7. Recombimant e6 protein for human papillomavirus type 18(HPV 18) E6 gene was expressed in E. coli in large quantity as authentic size, non fused form of protein.
However, the protein was produced in inclusion bodies. Therefore this bacterialy produced E6 protein was solublized in SDS solution and purified to the homogeneity by gel filtration column chromatographies using Sephacryl S-200 column. The yield
of
purified 18 E6 recombinant protein was approximately 30 mg/1 liter culture. This purified recombinant 18E6 protein was used to determine the pesence of antibodies that might have been produced against HPV 18 infections in the human sera. We
tested
and
compared sera obtained from both cervical cancer patients and people having no apparent ccrvical cnacers. These were accomplished using western blot. Antibodies reactive to recombinant HPV 18E6 protein were frequently detected in cases with
cervical
carcinoma(14 out of 20, 70%), compared to people with no apparent carcinoma(4 out of 9.44%0.
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